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Following incubation overnight, the culture was diluted 1:100 in Mueller-Hinton broth and 300 l applied to Whatman cyclopore circular membranes 0.2 m (Whatman International, Maidstone, UK) placed on Mueller Hinton agar and incubated at 37 C for 72 h

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Advertisement - Continue Reading Below 3 Marion Cotillard Getty Images November 2016 Be inspired by Cotillard's one-shoulder dress and embrace bright colours during pregnancy

Once a brave choice reserved for runway models and younger starlets, sheer dresses, naked illusion gowns, and barely-there pieces are the red carpet and Fashion Week trend of the season it appears