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2 at 25°C Suspend 20 gram powdered medium in 1 litre distilled water
amino acids
11 Na2-EDTA 4
A Phosphate buffer is present to inhibit acidification of the medium during cell growth
Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Iron Size:500g 2 at 25°C Suspend 20Ferric chloride, FeCl3, is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element. Ammonium sulphate, as a source of Nitrogen, has been omitted to test the ability of yeast cells to assimilate different nitrogen sources like Potassium nitrate. Suspend 1. 9 gram powdered medium in 1 litre distilled water Store dry at room
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